You want to remove the bottle off your trap when it is completely full. A trap like this, one that has some dead and some live bees in it, is a fantastic trap. This trap has bee sounds coming out and pheromones are being released. When bees die in the hole over Winter, other bees will come around in the Spring and they still smell the pheromones. When this happens, they will want to clean out the nest chamber and reuse it. So the best thing you can do is to leave your bottle on there until it is completely full.
Here is a bottle we just took off a bee trap — it is completely full and there are a few live ones piled in on top. A bottle like this holds about 200 bees. Now, if you want to use these dead bees for something, you can. You can put them in a new trap so the new trap smells like the pheromones — these things stink! And that pheromone, that smell of dead bees, will attract more bees to your trap. It makes it a bee magnet.